Fei Rui, Fei Yu, Zheng Sheng, Gao Yan-guang, Sun Hong-xia, Zeng Xian-lu
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
Acta Pharmacol Sin. 2008 Apr;29(4):499-506. doi: 10.1111/j.1745-7254.2008.00765.x.
To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves (PGBL) by inhibiting leucocyte adhesion.
The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber.
p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner.
p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.
通过抑制白细胞黏附来研究银杏叶多糖（PGBL）的抗炎机制。
分离并纯化粗制的PGBL。通过二甲苯诱导的耳部水肿和小鼠急性腹膜炎模型来检测纯化后的PGBL（p-PGBL）的抗炎作用。采用流式细胞术和流动腔研究p-PGBL对抑制P-选择素与其配体之间相互作用的影响。
p-PGBL可有效抑制小鼠的急性炎症，并在静态条件下干扰人白血病细胞系HL-60细胞或中性粒细胞与P-选择素的黏附，以及在流动条件下以剂量依赖方式干扰中性粒细胞与表达人P-选择素的中国仓鼠卵巢细胞和人脐静脉内皮细胞的黏附。
p-PGBL可通过干扰P-选择素与其配体之间的相互作用来抑制炎症过程。
