Direct electrochemistry of cytochrome c at ordered macroporous active carbon electrode.

Three-dimensionally (3D) ordered macroporous active carbon has been fabricated and used as electrode substrate for the direct electrochemistry of horse heart cytochrome c (Cyt c). The Cyt c immobilized on the surface of the ordered macroporous active carbon shows a pair of well-defined and nearly reversible redox waves at the formal potential of -0.033V in pH 6.8 phosphate buffer solution. The interaction between Cyt c and the 3D macroporous active carbon makes the formal potential shift negatively compared to that of Cyt c in solution. Spectrophotometric and electrochemical methods have been used to investigate the interaction between Cyt c and the porous active carbon. The immobilized Cyt c maintains its biological activity, and shows a surface controlled electrode process with the electron-transfer rate constant (k(s)) of 17.6s(-1) and the charge-transfer coefficient (a) of 0.52, and displays the features of a peroxidase in the electrocatalytic reduction of hydrogen peroxide (H(2)O(2)). A potential application of the Cyt c-immobilized porous carbon electrode as a biosensor to monitor H(2)O(2) has been investigated. The steady-state current response increases linearly with H(2)O(2) concentration from 2.0x10(-5) to 2.4x10(-4)moll(-1). The detection limit (3sigma) for determination of H(2)O(2) has been found to be 1.46x10(-5)moll(-1).