Zhang Lei, Zhang Jing, Zhang Chunhua
Department of Chemistry, College of Life and Environment Sciences, Shanghai Normal University, Guilin Road No. 100, Shanghai 200234, PR China.
Biosens Bioelectron. 2009 Mar 15;24(7):2085-90. doi: 10.1016/j.bios.2008.10.025. Epub 2008 Nov 8.
Polyaniline (PAN) nano-particles, nano-fibrils, and nano-network have been synthesized via electrochemical polymerization of aniline using a three-step electrochemical deposition procedure on alpha-alanine (ALA)-monolayer functionalized glassy carbon electrode (GCE). The structure and properties of PAN nano-structures have been characterized using field emission scanning electron microscope (SEM), Fourier transform infrared spectra (FT-IR), and electrochemical techniques. The 3-dimensional (3D) PAN nano-network/ALA composite film coated GCE (PAN-ALA/GCE) leads to the direct electrochemistry of horse heart cytochrome c (Cyt c) immobilized on this electrode surface. The immobilized Cyt c maintains its activity, showing a surface-controlled electrode process with the electron transfer rate constant (k(s)) of 21.9s(-1) and the charge-transfer coefficient (alpha) of 0.37, and could be used for the electrocatalytic reduction of hydrogen peroxide (H(2)O(2)). The steady-state current response increases linearly with H(2)O(2) concentration from 2.5 x 10(-5) to 3.0 x 10(-4) moll(-1). The detection limit (3delta) is 8.2 x 10(-6) moll(-1).
通过三步电化学沉积法，在α-丙氨酸（ALA）单层功能化玻碳电极（GCE）上对苯胺进行电化学聚合，合成了聚苯胺（PAN）纳米颗粒、纳米纤维和纳米网络。利用场发射扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）和电化学技术对PAN纳米结构的结构和性能进行了表征。三维（3D）PAN纳米网络/ALA复合膜包覆的GCE（PAN-ALA/GCE）实现了固定在该电极表面的马心细胞色素c（Cyt c）的直接电化学。固定化的Cyt c保持其活性，显示出表面控制的电极过程，电子转移速率常数（k(s)）为21.9s(-1)，电荷转移系数（α）为0.37，可用于过氧化氢（H(2)O(2)）的电催化还原。稳态电流响应随H(2)O(2)浓度从2.5×10(-5)到3.0×10(-4) moll(-1)呈线性增加。检测限（3δ）为8.2×10(-6) moll(-1)。
