转录终止：全力以赴。

Transcription termination: pulling out all the stops.

作者信息

Greenblatt Jack F

机构信息

Banting and Best Department of Medical Research and Department of Molecular Genetics, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON, Canada M5S 3E1.

出版信息

Cell. 2008 Mar 21;132(6):917-8. doi: 10.1016/j.cell.2008.03.003.

DOI:10.1016/j.cell.2008.03.003

PMID:18358802

Abstract

In this issue, Larson et al. (2008) describe the use of optical traps to pull on the DNA template or RNA transcript and thereby explore the termination mechanism for E. coli RNA polymerase at intrinsic terminators. Their results imply that, depending on the nature of the terminator sequence, RNA polymerase uses either hypertranslocation or RNA:DNA shearing to destabilize the hybrid in the transcription bubble.

摘要

在本期杂志中，拉尔森等人（2008年）描述了利用光镊拉动DNA模板或RNA转录本，从而探究大肠杆菌RNA聚合酶在固有终止子处的终止机制。他们的结果表明，根据终止子序列的性质，RNA聚合酶要么利用超易位，要么利用RNA:DNA剪切来破坏转录泡中的杂交体稳定性。