Terracio L, Rubin K, Gullberg D, Balog E, Carver W, Jyring R, Borg T K
Department of Anatomy, Cell Biology, University of South Carolina, Columbia 29208.
Circ Res. 1991 Mar;68(3):734-44. doi: 10.1161/01.res.68.3.734.
The interaction between components of the extracellular matrix and the cell surface of cardiac myocytes appears to be regulated in part by receptors belonging to the integrin superfamily. The expression of the integrins was investigated at different stages of development of the heart as well as during cardiac hypertrophy. The characterization of the membrane proteins showed that a beta 1-integrin and associated alpha-chains were responsible for the interaction with collagen, laminin, and fibronectin. Immunoprecipitation data indicated that the presence of specific alpha-chains varied with development. These data were correlated with the ability of the isolated myocytes to attach to specific components of the extracellular matrix. The expression of the alpha 1-chain was prominently associated with the recognition of interstitial collagens. The presence of the alpha 1-chain was also associated with stages when collagen synthesis was increased, especially during fetal and neonatal growth and cardiac hypertrophy. Immunohistochemical localization with the antiserum against beta 1-integrin demonstrated its specific localization near the Z lines of cardiac myocytes. The localization both in vitro and in vivo indicated that the beta 1-integrin may play a role in myofibrillogenesis during development. The present immunohistochemical, cell adhesion, and biochemical data clearly indicate that integrins play a major role in the regulation of the interaction between cardiac myocytes and the extracellular matrix during development and disease.
细胞外基质成分与心肌细胞表面之间的相互作用似乎部分受整合素超家族受体的调控。在心脏发育的不同阶段以及心肌肥大过程中，对整合素的表达进行了研究。膜蛋白的特性表明，β1整合素及相关的α链负责与胶原蛋白、层粘连蛋白和纤连蛋白的相互作用。免疫沉淀数据表明，特定α链的存在随发育而变化。这些数据与分离的心肌细胞附着于细胞外基质特定成分的能力相关。α1链的表达与间质胶原的识别显著相关。α1链的存在也与胶原合成增加的阶段相关，尤其是在胎儿期、新生儿期生长以及心肌肥大期间。用抗β1整合素抗血清进行免疫组织化学定位显示其在心肌细胞Z线附近的特定定位。体内外定位均表明，β1整合素可能在发育过程中的肌原纤维形成中发挥作用。目前的免疫组织化学、细胞黏附及生化数据清楚地表明，整合素在发育和疾病过程中对心肌细胞与细胞外基质之间相互作用的调控中起主要作用。
