Kuhn C, Lucius R, Matthes H F, Meusel G, Reich B, Kalinna B H
Department of Molecular Parasitology, Institute of Biology, Humboldt-University Berlin, Berlin, Germany.
Vet Parasitol. 2008 May 31;153(3-4):329-37. doi: 10.1016/j.vetpar.2008.02.007. Epub 2008 Feb 16.
Sarcoptic mange (or scabies) is an important skin disease which can affect a variety of species including humans, cattle, goats, sheep, horses, pigs, rabbits, and dogs. Approximately 300 million people are affected worldwide and in lifestock animals the infestation may lead to substantial economic losses caused by depression in growth and feed conversion rates. Diagnosis of Sarcoptes infestation is difficult and only a few serological tests have been developed using whole mite antigen for diagnosis of mange in animals. Here we describe the isolation and characterisation of cDNAs of several immunoreactive clones and their recombinant expression in Escherichia coli. Three of the proteins contain repetitive sequences which suggests that they might be involved in immune evasion. The application of these antigens in serodiagnosis and the suitability for diagnosis is discussed.
疥螨病（或疥疮）是一种重要的皮肤病，可影响包括人类、牛、山羊、绵羊、马、猪、兔和狗在内的多种物种。全球约有3亿人受到影响，在牲畜中，这种感染可能会因生长和饲料转化率下降而导致重大经济损失。疥螨感染的诊断很困难，目前仅开发了少数几种使用全螨抗原诊断动物疥螨病的血清学检测方法。在此，我们描述了几个免疫反应性克隆的cDNA的分离和表征及其在大肠杆菌中的重组表达。其中三种蛋白质含有重复序列，这表明它们可能参与免疫逃避。文中还讨论了这些抗原在血清诊断中的应用以及诊断的适用性。
