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IS53:一种用于丁香假单胞菌丁香致病变种分子分型的插入元件。

IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi.

作者信息

Quesada Jose M, Pérez-Martínez Isabel, Ramos Cayo, López María M, Penyalver Ramón

机构信息

Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera Km 4.5, 46113 Moncada, Valencia, Spain.

出版信息

Res Microbiol. 2008 Apr;159(3):207-15. doi: 10.1016/j.resmic.2007.12.010. Epub 2008 Feb 8.

Abstract

A worldwide collection of Pseudomonas savastanoi pv. savastanoi strains from olive knots was examined for the distribution, variation in position and copy numbers of the IS53 insertion element. Southern hybridization analysis of plasmid DNA from six olive strains using IS53 and repA probes revealed that this insertion element was present in the chromosomal replicon and not in a plasmid, as had been originally described in an oleander strain. Southern hybridization analysis also revealed that IS53 was present in multiple copies in all analyzed strains. Copy numbers of IS53 elements ranged from 4 up to 10. Although all strains displayed a remarkably high degree of restriction fragment length polymorphism, we demonstrated that transposition of this element is extremely rare in bacteria grown in vitro for up to 390 generations. The genetic diversity of 62 olive strains based on 47 different IS53 RFLP fingerprints and UPGMA analysis enabled all strains to be clustered into eight groups with 60% similarity. IS53 typing can be considered a suitable marker for epidemiological and ecological studies, given its widespread distribution on P. savastanoi pv. savastanoi olive populations, its high stability and the high degree of polymorphism generated.

摘要

对从橄榄瘤中分离得到的全球范围的野油菜黄单胞菌野油菜致病变种菌株进行了研究,以分析IS53插入元件的分布、位置变化和拷贝数。使用IS53和repA探针,对六个橄榄菌株的质粒DNA进行Southern杂交分析,结果表明,该插入元件存在于染色体复制子中,而非如最初在夹竹桃菌株中所描述的那样存在于质粒中。Southern杂交分析还显示,在所有分析的菌株中,IS53均以多拷贝形式存在。IS53元件的拷贝数范围为4至10。尽管所有菌株均表现出高度的限制性片段长度多态性,但我们证明,在体外培养达390代的细菌中,该元件的转座极为罕见。基于47种不同的IS53 RFLP指纹图谱和UPGMA分析,对62个橄榄菌株的遗传多样性进行分析,结果可将所有菌株聚类为八组,相似性为60%。鉴于IS53在野油菜黄单胞菌野油菜致病变种橄榄群体中的广泛分布、其高稳定性以及所产生的高度多态性,IS53分型可被视为流行病学和生态学研究的合适标记。

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