用随机扩增多态性 DNA 分析对突尼斯分离的假单胞菌 savastanoi pv. savastanoi 菌株进行分类。
Delineation of Pseudomonas savastanoi pv. savastanoi strains isolated in Tunisia by random-amplified polymorphic DNA analysis.
机构信息
Unité de Recherche Protection des Plantes Cultivées et Environnement, Institut de l'Olivier, Cité Mahrajène BP208 Tunis, Tunisia.
出版信息
J Appl Microbiol. 2009 Mar;106(3):886-94. doi: 10.1111/j.1365-2672.2008.04058.x. Epub 2009 Jan 21.
AIMS
To investigate the genetic diversity of Pseudomonas savastanoi pv. savastanoi strains and to look whether these strains were distributed to geographical location.
METHODS AND RESULTS
Random amplification of polymorphic DNA (RAPD) was used to discriminate between 58 Tunisian strains and 21 strains from various other countries of P. savastanoi pv. savastanoi, the causal agent of olive knot disease. Isolates were separated into three groups by cluster analysis and principal coordinate analysis of RAPD fingerprint data obtained with three primers (OPR-12, OPX-7 and OPX-14). Group 1 contained isolates from the southeast of Tunisia and European strains. Group 2 comprised strains isolated from the north of Tunisia exclusively while group 3 encompassed the majority of isolates obtained from five orchards located in the centre of Tunisia.
CONCLUSIONS
The results indicated that isolates of P. savastanoi pv. savastanoi were genetically distinct according to geographic regions. RAPD grouped isolates derived from the same orchard as identical.
SIGNIFICANCE AND IMPACT OF THE STUDY
This is the first application of RAPD in the delineation of P. savastanoi pv. savastanoi strains.
目的
研究丁香假单胞菌 pv. 丁香致病变种菌株的遗传多样性，并观察这些菌株是否分布于不同地理位置。
方法和结果
利用随机扩增多态性 DNA（RAPD）技术对 58 株来自突尼斯和 21 株来自其他国家的丁香假单胞菌 pv. 丁香致病变种菌株进行了区分，这些菌株是引起橄榄瘤疱病的病原菌。聚类分析和基于 3 个引物（OPR-12、OPX-7 和 OPX-14）获得的 RAPD 指纹数据的主坐标分析将分离物分为 3 组。第 1 组包含来自突尼斯东南部和欧洲的分离物。第 2 组仅包含来自突尼斯北部的分离物，而第 3 组包含了来自突尼斯中部五个果园的大多数分离物。
结论
结果表明，根据地理位置，丁香假单胞菌 pv. 丁香致病变种菌株的遗传特征存在明显差异。RAPD 将来自同一果园的分离物归为同一组。
意义和影响的研究
这是 RAPD 首次应用于丁香假单胞菌 pv. 丁香致病变种菌株的划分。
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