Lin Huan-jian, Yang Qin, Li Jing, Liu Ying, Wang Qi-yi
Department of Gastroenterology, People's Hospital of Guangdong Province, Guangzhou 510080, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Mar;28(3):436-7.
To investigate the effects of the two-valence vaccine consisting of Helicobacter pylori (H. pylori) catalase and urease subunit UreB in preventing H. pyloriinfection in mice.
C57BL/6 mice were divided into 7 groups and immunized with intragastric administration of catalase and UreB (both 100 microg) plus cholera toxin (CT, 2 microg), catalase (100 microg) plus CT (2 microg), UreB (100 microg) plus CT (2 microg), catalase (100 microg), UreB (100 microg), CT (2 microg), or PBS, respectively, once a week for 4 consecutive weeks. Two weeks after the last immunization, all the mice were challenged by live H. pylori, and sacrificed 4 weeks after the challenge to obtain the gastric mucosa samples for detecting H. pylori using semi-quantitative bacterial culture assay.
The total protection rate in mice immunized with the two-valence vaccine, single-valence vaccine of catalase, and single-valence vaccine of UreB was 83.3% (20/24), 41.7% (10/24) and 54.2% (13/24), respectively, and the rate in the other 4 groups were all 0. The H. pyloricolony density in mice with vaccination was significantly lower than that of other 4 groups (P<0.05). The total protection rate and H. pylori colony density differed significantly between the two-valence vaccination group and the single-valence vaccination groups (P<0.05).
The two-valence vaccine consisting of catalase, UreB and adjuvant has better immunoprotective effects than the single-valence vaccines.
研究由幽门螺杆菌（H. pylori）过氧化氢酶和脲酶亚基UreB组成的二价疫苗对小鼠幽门螺杆菌感染的预防作用。
将C57BL/6小鼠分为7组，分别通过胃内给予过氧化氢酶和UreB（均为100微克）加霍乱毒素（CT，2微克）、过氧化氢酶（100微克）加CT（2微克）、UreB（100微克）加CT（2微克）、过氧化氢酶（100微克）、UreB（100微克）、CT（2微克）或PBS进行免疫，每周1次，连续4周。末次免疫后2周，所有小鼠均用活的幽门螺杆菌进行攻击，攻击后4周处死小鼠，获取胃黏膜样本，采用半定量细菌培养法检测幽门螺杆菌。
用二价疫苗、过氧化氢酶单价疫苗和UreB单价疫苗免疫的小鼠总保护率分别为83.3%（20/24）、41.7%（10/24）和54.2%（13/24），其他4组的保护率均为0。接种疫苗小鼠的幽门螺杆菌菌落密度显著低于其他4组（P<0.05）。二价疫苗接种组与单价疫苗接种组的总保护率和幽门螺杆菌菌落密度差异有统计学意义（P<0.05）。
由过氧化氢酶、UreB和佐剂组成的二价疫苗比单价疫苗具有更好的免疫保护作用。
