[曲古抑菌素A对混合淋巴细胞培养中小鼠T细胞增殖及白细胞介素-2表达的影响]
[Effect of trichostatin A on proliferation and interleukin-2 expression of mouse T cells in mixed lymphocyte culture].
作者信息
Wei Qiang, Yu Li-xin, Deng Wen-feng, Ye Jun-sheng
机构信息
Department of Organ Transplantation, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Mar;28(3):467-9.
OBJECTIVE
To investigate the effect of trichostatin A (TSA) on proliferation and interleukin-2 (IL-2) expression of mouse T cells in mixed lymphocyte culture (MLC), and explore its effect on T cell-mediated immune response.
METHODS
BALB/c and C57BL mouse MLC was treated with different concentrations of TSA for different durations, and the lymphocyte inhibition ratio was measured by MTT assay. With CTX as the control, one-way MLC system of BALB/c and C57BL mouse was treated with the same concentrations of TSA, and IL-2 expression in the T cells was observed by flow cytometry.
RESULTS
TSA inhibited the proliferation of T cells in the MLC in a time- and dose-dependent fashion. It also reduced the IL-2 expression in one-way MLC dose-dependently, showing it significantly differed from the effect of CTX (P<0.01).
CONCLUSION
Histone deacetylase inhibitor TSA can inhibit the proliferation and reduce IL-2 expression of the T cells in MLC of mice, and therefore inhibit the T cell-mediated immune response.
目的
探讨曲古抑菌素A（TSA）对混合淋巴细胞培养（MLC）中小鼠T细胞增殖及白细胞介素-2（IL-2）表达的影响，并探讨其对T细胞介导的免疫反应的作用。
方法
用不同浓度的TSA处理BALB/c和C57BL小鼠的MLC不同时间，采用MTT法检测淋巴细胞抑制率。以环磷酰胺（CTX）为对照，用相同浓度的TSA处理BALB/c和C57BL小鼠的单向MLC系统，采用流式细胞术观察T细胞中IL-2的表达。
结果
TSA以时间和剂量依赖性方式抑制MLC中T细胞的增殖。它还以剂量依赖性方式降低单向MLC中IL-2的表达，表明其与CTX的作用有显著差异（P<0.01）。
结论
组蛋白去乙酰化酶抑制剂TSA可抑制小鼠MLC中T细胞的增殖并降低IL-2的表达，从而抑制T细胞介导的免疫反应。
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