Reynaud Yann, Saulnier Denis, Mazel Didier, Goarant Cyrille, Le Roux Frédérique
Laboratoire de Génétique et Pathologie, Ifremer, 17390 La Tremblade, France.
Appl Environ Microbiol. 2008 May;74(10):3038-47. doi: 10.1128/AEM.02680-07. Epub 2008 Mar 21.
Vibrio nigripulchritudo, the etiological agent of Litopenaeus stylirostris summer syndrome, is responsible for mass mortalities of shrimp in New Caledonia. Epidemiological studies led to the suggestion that this disease is caused by an emergent group of pathogenic strains. Genomic subtractive hybridization was carried out between two isolates exhibiting low and high virulence. Our subtraction library was constituted of 521 specific fragments; 55 of these were detected in all virulent isolates from our collection (n = 32), and 13 were detected only in the isolates demonstrating the highest pathogenicity (n = 19), suggesting that they could be used as genetic markers for high virulence capacity. Interestingly, 10 of these markers are carried by a replicon of 11.2 kbp that contains sequences highly similar to those of a plasmid detected in Vibrio shilonii, a coral pathogen. The detection of this plasmid was correlated with the highest pathogenicity status of the isolates from our collection. The origin and consequence of this plasmid acquisition are discussed.
黑丑弧菌是凡纳滨对虾夏季综合征的病原体，导致新喀里多尼亚虾类大量死亡。流行病学研究表明，这种疾病是由一群新出现的致病菌株引起的。对两株毒力低和高的分离株进行了基因组消减杂交。我们的消减文库由521个特异性片段组成；其中55个在我们收集的所有强毒株（n = 32）中都能检测到，13个仅在致病性最强的分离株（n = 19）中能检测到，这表明它们可作为高毒力的遗传标记。有趣的是，这些标记中有10个由一个11.2 kbp的复制子携带，该复制子包含与在珊瑚病原体希氏弧菌中检测到的一个质粒高度相似的序列。该质粒的检测与我们收集的分离株的最高致病状态相关。本文讨论了该质粒获得的起源和后果。
