Ogier-Denis E, Sapin C, Bauvy C, Hourri J J, Aubery M, Trugnan G, Codogno P
Unité INSERM 180, UFR Biomédicale des Saints-Pères, Paris.
C R Seances Soc Biol Fil. 1991;185(4):182-9.
HT-29 cells derived from a human colonic adenocarcinoma, can express a typical intestinal differentiation. Undifferentiated HT-29 cells accumulate N-linked glycoproteins substituted with unprocessed carbohydrate chains before to degrade them. Conversely, carbohydrate chains of N-linked glycoproteins are classically processed in differentiated HT-29 cells. The instability of N-linked glycoproteins in undifferentiated HT-29 cells is due to their rapid delivery from the endoplasmic reticulum to a compartment with lysosomal characteristics. This catabolitic pathway involves a bypass of the Golgi apparatus.
源自人结肠腺癌的HT-29细胞可表现出典型的肠道分化。未分化的HT-29细胞在降解N-连接糖蛋白之前会积累带有未加工碳水化合物链的N-连接糖蛋白。相反，N-连接糖蛋白的碳水化合物链在分化的HT-29细胞中会进行经典加工。未分化的HT-29细胞中N-连接糖蛋白的不稳定性是由于它们从内质网快速转运至具有溶酶体特征的区室。这种分解代谢途径涉及绕过高尔基体。
