[生长激素释放激素对GH3细胞钙摄取的刺激作用]
[Stimulation of calcium intake by growth-hormone-releasing-hormone in GH3 cells].
作者信息
Bresson L, Fahmi M, Sartor P, Mariot P, Dufy-Barbe L
机构信息
Laboratoire de Neurophysiologie, CNRS URA 1200, Université de Bordeaux II.
出版信息
C R Seances Soc Biol Fil. 1991;185(4):218-23.
The effect of GH-RH in the intra-cytosolic free Ca2+ concentration was studied in GH3 cells. To this end, we have used microspectrofluorimetry performed on single cells. We show that 60% of cells respond to a brief application of 100 nM GH-RH by an increase of their [Ca2+]i (mean increase 100% over basal values). This response which is blocked by calcium channel inhibitors results from an increased influx of Ca2+ ions from the external medium.
在GH3细胞中研究了生长激素释放激素（GH-RH）对细胞内游离钙离子（Ca2+）浓度的影响。为此，我们对单个细胞进行了显微分光荧光测定。我们发现，60%的细胞在短暂施加100 nM的GH-RH后，其细胞内钙离子浓度（[Ca2+]i）会升高（平均比基础值增加100%）。这种被钙通道抑制剂阻断的反应是由于细胞外介质中Ca2+离子内流增加所致。
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