Gedik Abdullah, Ersay Ahmet, Atmaca Selahattin, Sener Alper
Department of Urology, Diyarbakir Hospital, Urology Clinic, Diyarbakir, Turkey.
South Med J. 2008 Mar;101(3):236-9. doi: 10.1097/01.smj.0000305885.23903.71.
Oxybutynin is a tertiary amine and has chemical similarities like protamine sulfate. Protamine sulfate's effect on bacterial viability has been shown in some studies; however, there is scanty data regarding the effect of oxybutynin on bacterial viability. Accordingly, the aim of the present study was to investigate the effect of oxybutynin on bacterial viability.
Twenty of Staphylococcus epidermidis (S. epidermidis) strains were isolated from patients who had catheter-associated urinary tract infection. These strains were exposed to 5 different concentrations of oxybutynin in tryptic soy broth (TSB) media (max: 5 mg/mL, min: 0.05 mg/mL). Quantitative growth patterns were measured by spectrophotometer. Under the same media conditions, biofilm production of individual strains was measured by Deighton's micromethod at the end of the 6-hour incubation period.
After the incubation period, weak and strong slime-producing bacteria groups were obtained and both groups' slime productions were extensively low at 5 mg/mL concentration of oxybutynin. Slime production of S. epidermidis was inversely correlated with oxybutynin concentrations.
Although there was a dose-dependent reduction of biofilm production, there seemed to be no bactericidal effect of oxybutynin on S. epidermidis.
奥昔布宁是一种叔胺，与硫酸鱼精蛋白在化学性质上有相似之处。一些研究已表明硫酸鱼精蛋白对细菌活力的影响；然而，关于奥昔布宁对细菌活力影响的数据却很少。因此，本研究的目的是探究奥昔布宁对细菌活力的影响。
从患有导管相关性尿路感染的患者中分离出20株表皮葡萄球菌菌株。将这些菌株置于胰蛋白胨大豆肉汤（TSB）培养基中，暴露于5种不同浓度的奥昔布宁（最高浓度：5 mg/mL，最低浓度：0.05 mg/mL）。通过分光光度计测量定量生长模式。在相同的培养基条件下，在6小时孵育期结束时，采用戴顿微量法测量各菌株的生物膜形成情况。
孵育期结束后，获得了弱产黏液菌组和强产黏液菌组，在5 mg/mL浓度的奥昔布宁作用下，两组的黏液产生量均极低。表皮葡萄球菌的黏液产生量与奥昔布宁浓度呈负相关。
尽管生物膜形成量呈剂量依赖性减少，但奥昔布宁对表皮葡萄球菌似乎没有杀菌作用。
