Höinghaus R, Hewicker-Trautwein M, Mischke R
Small Animal Clinic, School of Veterinary Medicine Hannover, Hannover, Germany.
Vet Clin Pathol. 2008 Mar;37(1):104-11. doi: 10.1111/j.1939-165X.2008.00017.x.
Immunocytochemical techniques are a potentially valuable diagnostic tool to support cytologic diagnosis in dogs. However, detailed studies of staining patterns and intensity in cytologic specimens of mesenchymal tumor types are lacking.
The aim of this study was to evaluate commercially available antibodies against human proteins for use in the characterization of canine tumors of mesenchymal origin in cytologic samples.
Immunocytochemical staining was performed on air-dried imprint specimens of biopsies obtained from 103 mesenchymal neoplasms and 14 metastatic lesions from 98 dogs. All specimens were stained with anti-cytokeratin AE1/AE3 and vimentin. Based on the histologic diagnosis, tumors of muscle, endothelial, histiocytic, and melanocytic origin also were stained with cell-specific antibodies. Staining intensity was subjectively graded and the percentage of positive tumor cells was estimated.
All mesenchymal tumors and metastases, with the exception of mesotheliomas, were vimentin-positive and cytokeratin-negative; mesotheliomas (n=6) were positive for both vimentin and cytokeratin. Tumors of muscle (n=5), endothelial (n=15), and histiocytic (n=18) origin stained moderately to strongly positive in a majority of tumor cells with desmin, von Willebrand factor, and lysozyme, respectively. Malignant melanomas (n=15) had variable staining and a variable percentage of positive cells with Melan-A and S100.
Our results indicate that immunocytochemical staining of canine cytologic specimens is a reliable and sensitive technique that may be of benefit for the differentiation of poorly differentiated mesenchymal tumors and metastases. Additional study is needed to assess the specificity of immunocytochemical stains in mesenchymal tumors.
免疫细胞化学技术是一种潜在的有价值的诊断工具，可辅助犬类的细胞学诊断。然而，对于间充质肿瘤类型的细胞学标本的染色模式和强度缺乏详细研究。
本研究的目的是评估针对人类蛋白质的市售抗体，用于在细胞学样本中鉴定犬类间充质起源的肿瘤。
对从98只犬的103个间充质肿瘤和14个转移病灶获取的活检组织的空气干燥印片标本进行免疫细胞化学染色。所有标本均用抗细胞角蛋白AE1/AE3和波形蛋白染色。根据组织学诊断，肌肉、内皮、组织细胞和黑素细胞起源的肿瘤也用细胞特异性抗体染色。主观评估染色强度，并估计阳性肿瘤细胞的百分比。
除间皮瘤外，所有间充质肿瘤和转移灶波形蛋白呈阳性，细胞角蛋白呈阴性；间皮瘤（n=6）波形蛋白和细胞角蛋白均呈阳性。肌肉起源（n=5）、内皮起源（n=15）和组织细胞起源（n=18）的肿瘤在大多数肿瘤细胞中分别对结蛋白、血管性血友病因子和溶菌酶呈中度至强阳性染色。恶性黑色素瘤（n=15）对黑素-A和S100的染色及阳性细胞百分比各不相同。
我们的结果表明，犬类细胞学标本的免疫细胞化学染色是一种可靠且敏感的技术，可能有助于鉴别低分化间充质肿瘤和转移灶。需要进一步研究以评估免疫细胞化学染色在间充质肿瘤中的特异性。
