采用胶束电动毛细管色谱法测定食品、饮料、天然产物、药品和化妆品中的咖啡因及相关化合物。

Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography.

作者信息

Injac Rade, Srdjenovic Branislava, Prijatelj Matevz, Boskovic Marija, Karljikovic-Rajic Katarina, Strukelj Borut

机构信息

Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia.

出版信息

J Chromatogr Sci. 2008 Feb;46(2):137-43. doi: 10.1093/chromsci/46.2.137.

DOI:10.1093/chromsci/46.2.137

PMID:18366873

Abstract

A method is described for quantitating caffeine, theobromine, theophylline, paracetamol, propyphenazone, acetylsalicylic acid, salicylic acid, and codeine phosphate in corresponding real samples of food, beverages, natural products, pharmaceuticals, and cosmetic preparations by micellar electrokinetic capillary chromatography. The separation is carried out at 25 degrees C and 25 kV, using a 20 mM phosphate buffer (pH 9.0), 80 mM sodium dodecyl sulfate, and 7.5% (v/v) acetonitrile. UV detection is at 210 nm. The method is shown to be specific, accurate (recoveries over the range 98.9-101.2%), linear over the tested range (correlation coefficients>or=0.9993), and precise (relative standard deviation below 2.1%). The method is applied for the quantitative analysis of these compounds in different foods, beverages, natural products, pharmaceuticals, and cosmetic products.

摘要

描述了一种通过胶束电动毛细管色谱法定量分析食品、饮料、天然产物、药品和化妆品制剂等相应实际样品中咖啡因、可可碱、茶碱、对乙酰氨基酚、保泰松、乙酰水杨酸、水杨酸和磷酸可待因的方法。分离在25℃和25 kV下进行，使用20 mM磷酸盐缓冲液（pH 9.0）、80 mM十二烷基硫酸钠和7.5%（v/v）乙腈。紫外检测波长为210 nm。该方法具有特异性、准确性（回收率在98.9 - 101.2%范围内），在所测试范围内呈线性（相关系数≥0.9993），且精密度高（相对标准偏差低于2.1%）。该方法用于不同食品、饮料、天然产物、药品和化妆品中这些化合物的定量分析。