同时采用高效液相色谱法测定食品、饮料和草药产品中的咖啡因、可可碱和茶碱。
Simultaneous HPLC determination of caffeine, theobromine, and theophylline in food, drinks, and herbal products.
作者信息
Srdjenovic Branislava, Djordjevic-Milic Vukosava, Grujic Nevena, Injac Rade, Lepojevic Zika
机构信息
Medical Faculty, Department of Pharmacy, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia.
出版信息
J Chromatogr Sci. 2008 Feb;46(2):144-9. doi: 10.1093/chromsci/46.2.144.
A rapid and selective high-performance liquid chromatographic (HPLC) method is developed for the separation and determination of caffeine, theobromine, and theophylline. The chromatography is performed on a Zorbax Eclipse XDB-C8 column (4.6x150 mm i.d., 5-microm particle size) at 25 degrees C, with a mobile phase of water-THF (0.1% THF in water, pH 8)-acetonitrile (90:10, v/v). The flow rate is 0.8 mL/min, and detection is by UV at 273 nm. This method permits the simultaneous determination of caffeine, theobromine, and theophylline in food, drinks, and herbal products with detection limits of 0.07-0.2 mg/L and recoveries of 100.20-100.42%. Correlation coefficients, for the calibration curves in the linear range of 0.2-100 mg/L, are greater than 0.9999 for all compounds. The within- and between-day precision is determined for both retention times and peak area. The data suggests that the proposed HPLC method can be used for routine quality control of food, drinks, and herbal products.
建立了一种快速、选择性的高效液相色谱（HPLC）方法，用于分离和测定咖啡因、可可碱和茶碱。色谱分析在Zorbax Eclipse XDB-C8柱（内径4.6x150 mm，粒径5微米）上于25℃进行，流动相为水-四氢呋喃（水中含0.1%四氢呋喃，pH 8）-乙腈（90:10，v/v）。流速为0.8 mL/min，检测波长为273 nm的紫外光。该方法可同时测定食品、饮料和草药产品中的咖啡因、可可碱和茶碱，检测限为0.07-0.2 mg/L，回收率为100.20-100.42%。在0.2-100 mg/L线性范围内的校准曲线，所有化合物的相关系数均大于0.9999。测定了保留时间和峰面积的日内和日间精密度。数据表明，所提出的HPLC方法可用于食品、饮料和草药产品的常规质量控制。
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