Alexandre D, Chuchana P, Roncarolo M G, Yssel H, Spits H, Lefranc G, Lefranc M P
Laboratoire d'Immunogénétique Moléculaire, LIGM, Université Montpellier II, Sciences et Techniques du Languedoc, France.
Int Immunol. 1991 Oct;3(10):973-82. doi: 10.1093/intimm/3.10.973.
Novel variable (V)--joining (J) gene rearrangements are described in the human T cell receptor gamma locus, in which, on the one hand, the V3 variable gene is joined to the heptamer--nonamer recombination signals of the J1 segment and, on the other hand, the J1 segment is joined to the V3 recombination signals through head-to-head fusion. These recombination products, or hybrid joints, have been originated through an inversion of 47 kb DNA. Interestingly the inverted DNA stretch contains a normal V9-J9 rearrangement. These findings are the first direct demonstration that successive rearrangements occur, on the same chromosome, in the human T cell receptor gamma locus, and suggest that the chronology of the joining events plays a role in the ontogeny of T cells and their differentiation in gamma/delta + and alpha/beta + lineages.
在人类T细胞受体γ基因座中描述了新型可变（V）-连接（J）基因重排，其中，一方面，V3可变基因与J1区段的七聚体-九聚体重组信号相连，另一方面，J1区段通过头对头融合与V3重组信号相连。这些重组产物或杂种接头是通过47 kb DNA的倒位产生的。有趣的是，倒位的DNA片段包含正常的V9-J9重排。这些发现首次直接证明了在人类T细胞受体γ基因座的同一条染色体上发生了连续重排，并表明连接事件的时间顺序在T细胞的个体发生及其在γ/δ +和α/β +谱系中的分化中起作用。
