Hansen Dewey, Healy Mimi, Reece Kristy, Smith Cheryl, Woods Gail L
Associated Regional and University Pathologists, Inc., Infectious Diseases Laboratory, Salt Lake City, Utah, USA.
J Clin Microbiol. 2008 May;46(5):1835-9. doi: 10.1128/JCM.00956-07. Epub 2008 Mar 26.
Repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system was investigated for identification of Aspergillus. Ninety-five clinical isolates, identified by conventional methods, and five ATCC strains were tested. Sequencing of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) was performed on 2 isolates with discrepant rep-PCR and conventional results and 15 randomly selected outlier isolates. One isolate not identified by sequencing was excluded from analysis. After resolving discrepancies, all but one A. glaucus strain had >or=85% similarity to one or more strains of the same Aspergillus species in the mold database, thereby providing accurate identification. No isolate was misidentified.
研究了使用DiversiLab系统的基于重复序列的PCR(rep-PCR)用于鉴定曲霉。对95株通过传统方法鉴定的临床分离株和5株ATCC菌株进行了测试。对2株rep-PCR结果与传统结果不一致的分离株以及15株随机选择的异常分离株进行了内部转录间隔区(ITS)区域(ITS1-5.8S-ITS2)测序。将一株未通过测序鉴定的分离株排除在分析之外。解决差异后,除一株灰绿曲霉外,所有菌株与霉菌数据库中同一曲霉属的一种或多种菌株具有≥85%的相似性,从而提供了准确的鉴定。没有分离株被错误鉴定。