Koyama A, Irimajiri K
Third Department of Internal Medicine, Kinki University School of Medicine, Osaka.
Rinsho Ketsueki. 1991 Nov;32(11):1416-24.
We studied peripheral blood mononuclear cells, bone marrow cells and lymph node cells from 30 patients with non-Hodgkin's lymphoma by DNA hybridization. Clonal JH or TCR beta gene rearrangements were detected in peripheral blood or bone marrow mononuclear cells from 15 of 30 (50.0%) patients studied, including 31.8% of those without evidence of tumor cells by morphological method. Four of 9 cases demonstrated identical clonal gene rearrangements at diagnosis and in remission phase in peripheral blood mononuclear cells. The presence of clonal gene rearrangements roughly correlated with the clinical stage of disease. And the proportion of clonal rearrangements was higher in the groups with small & medium sized cell types (64.3%) than in the group with large cell type (33.3%). So, we concluded that Southern blot analysis was more useful than morphological method to detect monoclonality of circulating lymphoma cells and minimal tumor cells.
我们通过DNA杂交技术研究了30例非霍奇金淋巴瘤患者的外周血单核细胞、骨髓细胞和淋巴结细胞。在所研究的30例患者中的15例（50.0%）外周血或骨髓单核细胞中检测到克隆性JH或TCRβ基因重排，其中31.8%的患者通过形态学方法未发现肿瘤细胞证据。9例中的4例在诊断时及外周血单核细胞缓解期显示相同的克隆性基因重排。克隆性基因重排的存在与疾病的临床分期大致相关。中小细胞类型组的克隆重排比例（64.3%）高于大细胞类型组（33.3%）。因此，我们得出结论，Southern印迹分析在检测循环淋巴瘤细胞和微小肿瘤细胞的单克隆性方面比形态学方法更有用。
