Department of Physics, University of Kaiserslautern, 67663, Kaiserslautern, Germany.
Anal Bioanal Chem. 2008 May;391(2):545-54. doi: 10.1007/s00216-008-2014-z. Epub 2008 Mar 28.
The proteins lysozyme, amylase, and bovine serum albumin (BSA) were adsorbed on two experimental dental materials, made of fluoroapatite particles embedded in polymer matrices, and on silicon wafers. The protein films were prepared as single-component layers, as binary mixtures, and as double layers. These systems were investigated by time-of-flight secondary ion mass spectrometry (ToF-SIMS) and the multivariate data analysis technique of discriminant principal-component analysis (DPCA). During adsorption of a single protein film on to the solid surfaces, the three proteins could be clearly distinguished by the scores of their mass spectra after selection of amino acid-related peaks and DPCA. Furthermore, very similar results were obtained on the two different fluoroapatite substrates. For samples coated with binary layers of two proteins adsorbed simultaneously, it was found for both substrate types that BSA shows the strongest ability to adsorb followed by lysozyme, while amylase has the smallest ability. By contrast, the consecutive adsorption of two protein layers showed a strong influence of substrate type on the adsorption ability of the proteins.
溶菌酶、淀粉酶和牛血清白蛋白（BSA）这 3 种蛋白质被吸附在由氟磷灰石颗粒嵌入聚合物基质和硅片组成的两种实验性牙科材料上。将蛋白质薄膜制备为单一组分层、双组分混合物和双层。通过飞行时间二次离子质谱（ToF-SIMS）和判别主成分分析（DPCA）的多元数据分析技术对这些系统进行了研究。在单一蛋白质薄膜吸附到固体表面上时，通过选择与氨基酸相关的峰和 DPCA 对其质谱进行评分，可以清楚地区分这 3 种蛋白质。此外，在两种不同的氟磷灰石基质上得到了非常相似的结果。对于同时吸附两种蛋白质的双层样品，对于两种基质类型，都发现 BSA 具有最强的吸附能力，其次是溶菌酶，而淀粉酶的吸附能力最小。相比之下，两种蛋白质层的连续吸附显示出蛋白质的吸附能力强烈依赖于基底类型。
