Magavi Sanjay S, Macklis Jeffrey D
MGH-HMS Center for Nervous System Repair, Departments of Neurosurgery and Neurology, Program in Neuroscience, Harvard Medical School, Harvard University, Boston, MA, USA.
Methods Mol Biol. 2008;438:335-43. doi: 10.1007/978-1-59745-133-8_25.
Bromodeoxyuridine, variously abbreviated as BrdU, BudR, and BrdUrd, is a halogenated thymidine analog that is permanently integrated into the DNA of dividing cells during DNA synthesis in S phase. BrdU can be immunocytochemically detected in vitro and in vivo, allowing the identification of cells that were dividing the period of BrdU exposure. In vivo, it has been used to identify the "birthdate" of cells during development, to examine the fate of postnatally generated cells, and to label cells before transplantation, for subsequent identification.
溴脱氧尿苷，有多种缩写形式，如BrdU、BudR和BrdUrd，是一种卤代胸腺嘧啶类似物，在S期DNA合成过程中会永久整合到正在分裂的细胞的DNA中。BrdU可在体外和体内通过免疫细胞化学方法检测到，从而能够识别在BrdU暴露期间处于分裂状态的细胞。在体内，它已被用于确定发育过程中细胞的“出生日期”，研究出生后产生的细胞的命运，以及在移植前标记细胞以便后续识别。
