Nagamatsu Shinya, Ohara-Imaizumi Mica
Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan.
Methods Mol Biol. 2008;440:259-68. doi: 10.1007/978-1-59745-178-9_20.
The total internal reflection fluorescence (TIRF) technique is a powerful tool for visualizing the process of insulin exocytosis. This technique allows us to observe the events just beneath the plasma membrane, allowing us to explore the molecular mechanism of insulin exocytotic processes such as docking and fusion. In this review, we describe the method of TIRF for visualizing insulin exocytosis in pancreatic beta-cells.
全内反射荧光（TIRF）技术是用于可视化胰岛素胞吐过程的有力工具。该技术使我们能够观察质膜下方的事件，从而探索胰岛素胞吐过程（如对接和融合）的分子机制。在本综述中，我们描述了用于可视化胰腺β细胞中胰岛素胞吐的TIRF方法。
