Schleifman Erica B, Chin Joanna Y, Glazer Peter M
Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.
Methods Mol Biol. 2008;435:175-90. doi: 10.1007/978-1-59745-232-8_13.
Gene targeting with DNA-binding molecules such as triplex-forming oligonucleotides or peptide nucleic acids can be utilized to direct mutagenesis or induce recombination site-specifically. In this chapter, several detailed protocols are described for the design and use of triplex-forming molecules to bind and mediate gene modification at specific chromosomal targets. Target site identification, binding molecule design, as well as various methods to test binding and assess gene modification are described.
利用诸如三链形成寡核苷酸或肽核酸等DNA结合分子进行基因靶向，可以特异性地指导诱变或诱导重组。在本章中，将描述几个详细的方案，用于设计和使用三链形成分子，以在特定染色体靶点处结合并介导基因修饰。文中描述了靶点识别、结合分子设计，以及测试结合和评估基因修饰的各种方法。
