Santos Anésia A, Florentino Lilian H, Pires Acássia B L, Fontes Elizabeth P B
Departamento de Bioquímica e Biologia Molecular, BIOAGRO- Universidade Federal de Viçosa-36571.000, Viçosa, MG, Brazil.
Methods Mol Biol. 2008;451:563-79. doi: 10.1007/978-1-59745-102-4_39.
The Geminiviridae family is a large family of plant viruses that has single-stranded DNA genomes and infects a large variety of crop species. In this chapter, we describe a biolistic inoculation protocol that has been successfully used to propagate new species of geminivirus in permissive hosts with total DNA extracted from infected plants. This allows us to directly investigate the biological properties of uncloned and not sap-transmissible geminiviruses.
双生病毒科是一类大型植物病毒家族，其基因组为单链DNA，可感染多种作物品种。在本章中，我们描述了一种生物弹道接种方案，该方案已成功用于利用从受感染植物中提取的总DNA在允许的宿主中繁殖双生病毒的新物种。这使我们能够直接研究未克隆且不能通过汁液传播的双生病毒的生物学特性。
