Dulhunty A F, Junankar P R, Stanhope C
Division of Neuroscience, John Curtin School of Medical Research, ANU, Canberra City, Australia.
Neurosci Lett. 1991 Oct 14;131(2):187-92. doi: 10.1016/0304-3940(91)90610-6.
The density of Mg(2+)-dependent Ca2+ ATPase in the terminal cisternae of pig skeletal muscle fibers was investigated to discover whether a reduction in Ca2+ ATPase content impairs Ca2+ sequestration and contributes to the elevated myoplasmic Ca2+ concentration in malignant hyperthermia. Unexpectedly, immunogold electron microscopy showed an increase in Ca2+ ATPase, while densitometry of SDS-polyacrylamide gels suggested that the Ca2+ ATPase content of terminal cisternae vesicles did not change. The affinity of Ca2+ ATPase in vesicles for our monoclonal antibody was not altered. We suggest that the availability of antigenic sites in malignant hyperthermia increases after processing for electron microscopy, perhaps as a consequence of altered sarcoplasmic reticulum membrane properties.
研究猪骨骼肌纤维终末池内Mg(2+)依赖的Ca2+ ATP酶的密度，以确定Ca2+ ATP酶含量的降低是否会损害Ca2+的隔离，并导致恶性高热时肌浆Ca2+浓度升高。出乎意料的是，免疫金电子显微镜显示Ca2+ ATP酶增加，而SDS-聚丙烯酰胺凝胶的光密度测定表明终末池小泡的Ca2+ ATP酶含量没有变化。小泡中Ca2+ ATP酶对我们单克隆抗体的亲和力没有改变。我们认为，在进行电子显微镜处理后，恶性高热中抗原位点的可用性增加，这可能是肌浆网细胞膜特性改变的结果。
