van Passel Mark W J
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB, Wageningen, The Netherlands.
Biol Direct. 2008 Mar 27;3:12. doi: 10.1186/1745-6150-3-12.
Most sequenced strains from Pasteurellaceae and Neisseriae contain hundreds to thousands of uptake sequence (US) motifs in their genome, which are associated with natural competence for DNA uptake. The mechanism of their recognition is still unclear, and I searched for intragenic location patterns of these motifs for clues about their distribution. In all cases, one orientation of the US has a higher occurrence in the reading frame, and in all Pasteurellaceae, the US and the reverse complement motifs are biased towards the gene termini. These findings could help design experimental set-ups to study preferential DNA uptake, thereby further unravelling the phenomenon of natural competence.
巴斯德氏菌科和奈瑟氏菌科的大多数测序菌株在其基因组中含有数百到数千个摄取序列（US）基序，这些基序与DNA摄取的自然感受态有关。它们的识别机制仍不清楚，我研究了这些基序的基因内定位模式，以寻找有关其分布的线索。在所有情况下，US的一个方向在阅读框中的出现频率更高，并且在所有巴斯德氏菌科中，US和反向互补基序都偏向于基因末端。这些发现有助于设计实验装置来研究优先DNA摄取，从而进一步揭示自然感受态现象。
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