Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
Institute for Animal Hygiene and Veterinary Public Health, University of Leipzig, Leipzig, Germany.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2424-2430. doi: 10.1099/ijs.0.062786-0. Epub 2014 Apr 28.
Five bacterial strains isolated from bats of the family Vespertilionidae were characterized by phenotypic tests and multilocus sequence analysis (MLSA) using the 16S rRNA gene and four housekeeping genes (rpoA, rpoB, infB, recN). Phylogenetic analyses of individual and combined datasets indicated that the five strains represent a monophyletic cluster within the family Pasteurellaceae. Comparison of 16S rRNA gene sequences demonstrated a high degree of similarity (98.3-99.9%) among the group of bat-derived strains, while searches in nucleotide databases indicated less than 96% sequence similarity to known members of the Pasteurellaceae. The housekeeping genes rpoA, rpoB, infB and recN provided higher resolution compared with the 16S rRNA gene and subdivided the group according to the bat species from which the strains were isolated. Three strains derived from noctule bats shared 98.6-100% sequence similarity in all four genes investigated, whereas, based on rpoB, infB and recN gene sequences, 91.8-96% similarity was observed with and between the remaining two strains isolated from a serotine bat and a pipistrelle bat, respectively. Genome relatedness as deduced from recN gene sequences correlated well with the results of MLSA and indicated that the five strains represent a new genus. Based on these results, it is proposed to classify the five strains derived from bats within Vespertiliibacter pulmonis gen. nov., sp. nov. (the type species), Vespertiliibacter genomospecies 1 and Vespertiliibacter genomospecies 2. The genus can be distinguished phenotypically from recognized genera of the Pasteurellaceae by at least three characteristics. All strains are nutritionally fastidious and require a chemically defined supplement with NAD for growth. The DNA G+C content of strain E127/08(T) is 38.2 mol%. The type strain of Vespertiliibacter pulmonis gen. nov., sp. nov. is E127/08(T) ( = CCUG 64585(T) = DSM 27238(T)). The reference strains of Vespertiliibacter genomospecies 1 and 2 are E145/08 and E157/08, respectively.
从蝙蝠科蝙蝠中分离出的 5 株细菌通过表型试验和多位点序列分析（MLSA），使用 16S rRNA 基因和 4 个管家基因（rpoA、rpoB、infB、recN）进行了鉴定。个体和组合数据集的系统发育分析表明，这 5 株细菌在巴斯德氏菌科内代表一个单系群。16S rRNA 基因序列比较表明，蝙蝠来源菌株之间具有高度相似性（98.3-99.9%），而核苷酸数据库搜索表明，与巴斯德氏菌科的已知成员的序列相似性小于 96%。管家基因 rpoA、rpoB、infB 和 recN 与 16S rRNA 基因相比提供了更高的分辨率，并根据分离株的蝙蝠种类对该组进行了细分。从夜蝠中分离出的 3 株细菌在所有 4 个基因中共享 98.6-100%的序列相似性，而基于 rpoB、infB 和 recN 基因序列，与从 serotine 蝙蝠和 pipistrelle 蝙蝠中分离出的另外 2 株细菌之间观察到 91.8-96%的相似性。根据 recN 基因序列推断的基因组相关性与 MLSA 的结果很好地吻合，表明这 5 株细菌代表一个新属。基于这些结果，建议将从蝙蝠中分离出的 5 株细菌分类为 Vespertiliibacter pulmonis gen. nov.，sp. nov.（模式种）、Vespertiliibacter 基因组种 1 和 Vespertiliibacter 基因组种 2。该属在表型上可与巴斯德氏菌科中公认的属区分开来，至少有三个特征。所有菌株在营养上都很挑剔，需要含有 NAD 的化学成分明确的补充剂才能生长。菌株 E127/08(T)的 DNA G+C 含量为 38.2 mol%。Vespertiliibacter pulmonis gen. nov.，sp. nov.的模式菌株是 E127/08(T)（=CCUG 64585(T)=DSM 27238(T)）。Vespertiliibacter 基因组种 1 和 2 的参考菌株分别是 E145/08 和 E157/08。
