基于高锰酸钾-钙黄绿素化学发光体系测定芬氟拉明

Determination of fenfluramine based on potassium permanganate-calcein chemiluminescence system.

作者信息

Nie Fei, Lu Jiuru

机构信息

School of Chemistry and Material Science, Shaanxi Normal University, Xi'an 710062, China.

出版信息

Talanta. 2008 Feb 15;74(5):1242-6. doi: 10.1016/j.talanta.2007.08.042. Epub 2007 Sep 8.

DOI:10.1016/j.talanta.2007.08.042

PMID:18371776

Abstract

Calcein was found to be able to use as chemiluminescence reagent and post-chemiluminescence was observed when fenfluramine was injected into the mixture after the CL reaction of calcein-potassium permanganate. Based on this phenomenon, a flow injection CL method was established for the determination of fenfluramine. The possible CL mechanism was proposed. The CL intensity was correlated linearly with the concentration of fenfluramine over the range of 1.0 x 10(-7) to 6.0 x 10(-6) g mL(-1) and the detection limit was 6 x 10(-8) g mL(-1). The relative standard deviation was 2.2% for 5.0 x 10(-7) g mL(-1) fenfluramine (n=11). This method was applied to the determination of fenfluramine in weight-reducing tonic successfully.

摘要

发现钙黄绿素可用作化学发光试剂，在钙黄绿素 - 高锰酸钾的化学发光反应后，向混合物中注入芬氟拉明时观察到后化学发光现象。基于此现象，建立了流动注射化学发光法测定芬氟拉明。提出了可能的化学发光机理。在1.0×10⁻⁷至6.0×10⁻⁶ g mL⁻¹范围内，化学发光强度与芬氟拉明浓度呈线性关系，检测限为6×10⁻⁸ g mL⁻¹。对于5.0×10⁻⁷ g mL⁻¹的芬氟拉明，相对标准偏差为2.2%（n = 11）。该方法成功应用于减肥滋补品中芬氟拉明的测定。