Fei Nie, Jiuru Lu
School of Chemistry and Material Science, Shaanxi Normal University, Xi'an 710062, PR China.
Anal Chim Acta. 2007 Jun 5;592(2):168-72. doi: 10.1016/j.aca.2007.04.041. Epub 2007 May 3.
Chemiluminescence (CL) was observed when potassium hexacyanoferrate(III) reacted with the mixture of calcein and ketotifen. Interestingly, the CL intensity would be enhanced by trace amounts of Mg2+ and the CL intensity was strongly dependent on ketotifen concentration. Based on this phenomenon, a flow injection CL method was established for the determination of ketotifen. The possible CL mechanism is proposed based on the kinetic characteristic of the CL reaction, CL spectrum, ultraviolet (UV) spectra and fluorescent spectra. The CL intensity was correlated linearly with concentration of ketotifen over the range of 6.0x10(-9) to 2.0x10(-7) g mL(-1) and the detection limit was 3x10(-9) g mL(-1). The relative standard deviation was 1.8% for 2.0x10(-8) g mL(-1) ketotifen (n=11). This method was applied to the determination of ketotifen in the tablets successfully.
当铁氰化钾与钙黄绿素和酮替芬的混合物反应时会观察到化学发光现象。有趣的是，痕量的Mg2+会增强化学发光强度，且化学发光强度强烈依赖于酮替芬浓度。基于此现象，建立了一种流动注射化学发光法用于测定酮替芬。根据化学发光反应的动力学特征、化学发光光谱、紫外光谱和荧光光谱，提出了可能的化学发光机理。在6.0×10(-9)至2.0×10(-7) g mL(-1)范围内，化学发光强度与酮替芬浓度呈线性关系，检测限为3×10(-9) g mL(-1)。对于2.0×10(-8) g mL(-1)的酮替芬，相对标准偏差为1.8%（n = 11）。该方法成功应用于片剂中酮替芬的测定。
