用于采用三氯乙酸沉淀法的流行病学研究的血浆抗坏血酸的制备与储存
Plasma ascorbic acid preparation and storage for epidemiological studies using TCA precipitation.
作者信息
Salminen Irma, Alfthan Georg
机构信息
Department of Health and Functional Capacity, Biomarker Laboratory, National Public Health Institute, Helsinki, Finland.
出版信息
Clin Biochem. 2008 Jun;41(9):723-7. doi: 10.1016/j.clinbiochem.2007.01.026. Epub 2007 Jun 21.
OBJECTIVES
To introduce a procedure to validate an ascorbic acid method using trichloroacetic acid (TCA) for plasma stabilization at different storage temperatures.
METHODS
EDTA and heparin plasma were precipitated with TCA (1:5) containing 0.54 mol/L EDTA, or without. Samples were stored at -20 degrees C and -70 degrees C and their stability was tested at room temperature for 24 h.
RESULTS
A significant 40% loss (p<0.001) of plasma ascorbic acid was found when EDTA samples with added EDTA were stored at -20 degrees C for 2-4 weeks compared with storage at -70 degrees C. Ascorbic acid in heparin plasma without added EDTA was most unstable and samples left at room temperature for 24 h lead to almost a total loss of ascorbic acid. Addition of EDTA to the TCA solution improved stability of samples of both plasma types at room temperature.
CONCLUSION
The recommended procedure for ascorbic acid determination in plasma stabilized with TCA is immediate storage at -70 degrees C and inclusion of EDTA into the TCA solution.
目的
介绍一种验证使用三氯乙酸（TCA）在不同储存温度下稳定血浆的抗坏血酸方法的程序。
方法
用含有0.54 mol/L EDTA的TCA（1:5）或不用其沉淀乙二胺四乙酸（EDTA）和肝素血浆。样品储存在-20℃和-70℃，并在室温下测试其稳定性24小时。
结果
与储存在-70℃相比，添加EDTA的EDTA样品在-20℃储存2-4周时，血浆抗坏血酸显著损失40%（p<0.001）。未添加EDTA的肝素血浆中的抗坏血酸最不稳定，在室温下放置24小时的样品导致抗坏血酸几乎完全损失。向TCA溶液中添加EDTA提高了两种血浆类型样品在室温下的稳定性。
结论
用TCA稳定血浆中抗坏血酸测定的推荐程序是立即储存在-70℃并将EDTA加入TCA溶液中。
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