Mohri M, Moosavian H R, Hadian M J
Department of Clinical Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Res Vet Sci. 2008 Dec;85(3):554-8. doi: 10.1016/j.rvsc.2008.02.009. Epub 2008 Mar 26.
The effects of various types of anticoagulants on plasma biochemistry were studied in man and various animals but limited informations are existing for camel plasma biochemistry. Eleven clinically healthy one-humped camels were blood sampled in different tubes containing different anticoagulants and plain tubes for harvesting plasma and serum. The concentrations of glucose, cholesterol, triglyceride, total bilirubin, urea, creatinine, total protein, albumin, calcium, inorganic phosphorus, magnesium, and chloride and the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), creatine kinase (CK) and gamma glutamyl transferase (GGT) were measured. Except for the amounts of AST, ALT, CK, total bilirubin, and inorganic phosphorus, other measured parameters were significantly lower in citrated plasma than in serum. Most parameters did not show any difference, but significant increase for CK activity and significant decrease for GGT, cholesterol, creatinine and chloride were seen when heparin was used as anticoagulant. Using EDTA as an anticoagulant caused a significant difference in the amounts of some measured parameters in plasma except glucose, AST, ALT, GGT, cholesterol, albumin, total protein, bilirubin, and triglyceride in comparison with serum.
在人类和各种动物中研究了不同类型抗凝剂对血浆生物化学的影响,但关于骆驼血浆生物化学的信息有限。对11只临床健康的单峰骆驼进行采血,分别采集到含有不同抗凝剂的不同试管以及用于获取血浆和血清的普通试管中。测定了葡萄糖、胆固醇、甘油三酯、总胆红素、尿素、肌酐、总蛋白、白蛋白、钙、无机磷、镁、氯的浓度以及天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)、肌酸激酶(CK)和γ-谷氨酰转移酶(GGT)的活性。除AST、ALT、CK、总胆红素和无机磷的含量外,枸橼酸盐血浆中其他测定参数均显著低于血清。大多数参数没有显示出任何差异,但当使用肝素作为抗凝剂时,CK活性显著增加,GGT、胆固醇、肌酐和氯显著降低。与血清相比,使用乙二胺四乙酸(EDTA)作为抗凝剂会导致血浆中某些测定参数的含量出现显著差异,但葡萄糖、AST、ALT、GGT、胆固醇、白蛋白、总蛋白、胆红素和甘油三酯除外。