Sacchetto Emilie, Ali Damien, Dumontet Erwan, Le Carrer Didier, Orsonneau Jean-Luc, Delaroche Odile, Bigot-Corbel Edith
Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
Ann Biol Clin (Paris). 2014 May-Jun;72(3):337-50. doi: 10.1684/abc.2014.0959.
The study of the influence of the anticoagulant used in blood collection tubes to obtain plasma was performed for fifteen biochemical parameters measured with automated Cobas 6000 (Roche Diagnostics). For each parameter tested the entire measurement domain was studied. The comparison of results obtained on plasma blood sample obtained by lithium heparin and EDTA include: correlation, the limits of acceptability in the standards of monitoring and interpretation standards regression defined by the SFBC and analysis of Bland-Altman. The parameters studied were classified into three categories. The parameters for which the assay is not influenced by the nature of the anticoagulant used: apolipoprotéin A1, apolipoprotein B, alanine amino-transferase, creatine kinase, creatinine, total cholesterol, HDL-cholesterol, lipase, NT-Pro BNP, troponine T and urea. The parameters for which the results are underestimated EDTA plasma, including those for which the impact is moderate and for which the interpretive standards are not changed: triglycerides, and those for which performance standards are changed on one or more levels: aspartate aminotransferase and lactate dehydrogenase; and finally the not practicable EDTA plasma parameters: alkaline phosphatase.
我们针对使用采血管中抗凝剂获取血浆时对15项生化参数的影响展开了研究，这些参数通过全自动Cobas 6000（罗氏诊断）进行测量。对于每项测试参数，我们研究了整个测量范围。对通过肝素锂和乙二胺四乙酸（EDTA）获得的血浆血样的结果进行比较，包括：相关性、监测标准中的可接受限度以及由法国生物临床学会（SFBC）定义的解释标准回归分析和布兰德-奥特曼分析。所研究的参数分为三类。检测不受所用抗凝剂性质影响的参数有：载脂蛋白A1、载脂蛋白B、丙氨酸氨基转移酶、肌酸激酶、肌酐、总胆固醇、高密度脂蛋白胆固醇、脂肪酶、N末端脑钠肽前体、肌钙蛋白T和尿素。EDTA血浆会使结果被低估的参数，包括影响程度适中且解释标准未改变的参数：甘油三酯，以及在一个或多个水平上性能标准发生改变的参数：天冬氨酸氨基转移酶和乳酸脱氢酶；最后是不适用EDTA血浆的参数：碱性磷酸酶。
