Benson A P, Christensen V L, Fairchild B D, Davis A J
Department of Poultry Science, University of Georgia, Athens, 30602-2772, USA.
Anim Reprod Sci. 2009 Apr;111(2-4):149-59. doi: 10.1016/j.anireprosci.2008.02.013. Epub 2008 Feb 23.
The avian inner perivitelline layer (IPVL) contains zona pellucida protein-B1 (ZPB1), zona pellucida protein-C (ZPC) and zona pellucida protein-D (ZPD). These three proteins may be involved in sperm binding to the IPVL. ZPB1 is produced by the liver and transported to the developing preovulatory follicle, while ZPC and ZPD are synthesized and secreted by the granulosa cells of the preovulatory follicle. The mRNA of ZPB1, ZPC, and ZPD was investigated in two lines of turkey hens selected for over 40 generations for either increased egg production (E line) or increased body weight (F line). Total RNA was extracted from the liver and from 1cm(2) sections of the granulosa layer around the germinal disc and a nongerminal disc area of the F(1) and F(2) follicles of hens from each genetic line. Northern analysis was performed using chicken cDNA probes for all three ZP proteins. Hepatic mRNA for ZPB1 was greater (P<0.05) in turkey hens from the E line than the F line. Although, there was no difference in ZPC mRNA between the germinal disc and nongerminal disc region of the two largest follicles in E line hens, ZPC mRNA was greater in the nongerminal disc region compared to the germinal disc region in the two largest follicles obtained from the F line hens. There were no differences in ZPD mRNA between the germinal disc and nongerminal disc regions of the F(1) and F(2) follicles for either genetic line. The results suggest that the greater rates of fertility previously observed in eggs from the E line hens compared with the F line of hens may be related to differential amounts of the potential sperm binding proteins ZPB1 and ZPC.
禽类卵黄膜内层（IPVL）包含透明带蛋白-B1（ZPB1）、透明带蛋白-C（ZPC）和透明带蛋白-D（ZPD）。这三种蛋白质可能参与精子与IPVL的结合。ZPB1由肝脏产生并转运至发育中的排卵前卵泡，而ZPC和ZPD则由排卵前卵泡的颗粒细胞合成并分泌。在经过40多代选育的两个火鸡品系中，对ZPB1、ZPC和ZPD的mRNA进行了研究，一个品系为产蛋量增加（E系），另一个品系为体重增加（F系）。从每个遗传品系母鸡的肝脏以及F1和F2卵泡胚盘周围和非胚盘区域的颗粒层1cm²切片中提取总RNA。使用针对所有三种ZP蛋白的鸡cDNA探针进行Northern分析。E系火鸡母鸡肝脏中ZPB1的mRNA含量高于F系（P<0.05）。虽然E系母鸡两个最大卵泡的胚盘和非胚盘区域之间ZPC mRNA没有差异，但F系母鸡两个最大卵泡的非胚盘区域ZPC mRNA含量高于胚盘区域。对于任何一个遗传品系，F1和F2卵泡的胚盘和非胚盘区域之间ZPD mRNA均无差异。结果表明，与F系母鸡相比，之前在E系母鸡所产鸡蛋中观察到的较高受精率可能与潜在精子结合蛋白ZPB1和ZPC的含量差异有关。
