Mercader Josep V, Suárez-Pantaleón Celia, Agulló Consuelo, Abad-Somovilla Antonio, Abad-Fuentes Antonio
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, P.O. Box 73, 46100 Burjassot, València, Spain.
J Agric Food Chem. 2008 Apr 23;56(8):2581-8. doi: 10.1021/jf800157s. Epub 2008 Mar 29.
High-affinity and selective monoclonal antibodies have been produced against the strobilurin fungicide trifloxystrobin. A battery of functionalized haptens has been synthesized, and conjugate-coated enzyme-linked immunosorbent assays following different procedures have been developed. On the one hand, a two-step conjugate-coated immunoassay was optimized using extended or short incubation times, with limits of detection of 0.10 ng/mL for the extended assay and 0.17 ng/mL for the rapid assay. On the other hand, an immunoassay in the conjugate-coated format was optimized following a procedure consisting of just one incubation step. This one-step assay had a limit of detection of 0.21 ng/mL. All of these assays showed detection limits for trifloxystrobin in the low parts per billion range, well below the common maximum residue limits for this pesticide in foodstuffs (50 microg/kg).
已制备出针对甲氧基丙烯酸酯类杀菌剂肟菌酯的高亲和力和选择性单克隆抗体。合成了一系列功能化半抗原，并开发了遵循不同程序的偶联物包被酶联免疫吸附测定法。一方面，使用延长或缩短的孵育时间优化了两步偶联物包被免疫测定法，延长测定法的检测限为0.10 ng/mL，快速测定法的检测限为0.17 ng/mL。另一方面，按照仅包含一个孵育步骤的程序优化了偶联物包被形式的免疫测定法。这种一步测定法的检测限为0.21 ng/mL。所有这些测定法显示肟菌酯的检测限在十亿分之几的低范围内，远低于该农药在食品中的常见最大残留限量（50 μg/kg）。
