在四氧嘧啶诱导的糖尿病大鼠中，成功的胰腺移植与眼部并发症控制之间的时间关系。

Temporal relationship between successful pancreas transplantation and control of ocular complications in alloxan-induced diabetic rats.

作者信息

Spadella C T, Machado J L M, Lerco M M, Ortolan E V P, Schellini S A, Gregório E A

机构信息

Department of Surgery, Botucatu School of Medicine, São Paulo State University (Unesp), Botucatu, SP, Brazil.

出版信息

Transplant Proc. 2008 Mar;40(2):518-23. doi: 10.1016/j.transproceed.2008.01.049.

DOI:10.1016/j.transproceed.2008.01.049

PMID:18374119

Abstract

PURPOSE

The impact of pancreas transplantation (PT) on the progression of eye disease is still controversial. This study evaluated the course of retinopathy in transplanted rats in two different diabetic stages.

METHODS

Sixty inbred male Lewis rats were assigned to four experimental groups: NC-15 nondiabetic control rats; DC-15 untreated diabetic control rats; PT1-15 diabetic rats that received syngeneic pancreas transplants 2 weeks after alloxan diabetes induction; PT2-15 diabetic rats that received pancreas transplants 12 weeks after diabetes onset. Clinical and laboratory parameters and lens opacity were examined in all rats prior to treatment and at 1-, 6-, and 12-months follow-up. Nucleated eyes from five rats in each group processed for ultrastructural study of the retinal at 6 and 12 months after PT or at follow-up.

RESULTS

Cataracts were observed in 20%, 60%, and 100% of DC rats at 1-, 6-, and 12-months follow-up, respectively. Early PT (2 weeks) significantly reduced the prevalence of this complication but not late (12 weeks) PT. PT1 rats also showed improved ultrastructure of the superficial and deep capillary plexuses of the retina, and of Müller cells, compared with DC and PT2. In the last group, retinopathy continued to evolve despite successful PT.

CONCLUSION

Our results suggested that prevention of diabetic ocular lesions by PT was closely dependent on earlier performance of the procedure.

摘要

目的

胰腺移植（PT）对眼部疾病进展的影响仍存在争议。本研究评估了处于两种不同糖尿病阶段的移植大鼠视网膜病变的病程。

方法

将60只近交系雄性Lewis大鼠分为四个实验组：NC - 15只非糖尿病对照大鼠；DC - 15只未治疗的糖尿病对照大鼠；PT1 - 15只糖尿病大鼠，在四氧嘧啶诱导糖尿病2周后接受同基因胰腺移植；PT2 - 15只糖尿病大鼠，在糖尿病发病12周后接受胰腺移植。在治疗前以及随访1个月、6个月和12个月时，对所有大鼠的临床和实验室参数以及晶状体混浊情况进行检查。每组选取5只大鼠的有核眼，在胰腺移植后6个月和12个月或随访时进行视网膜超微结构研究。

结果

在随访1个月、6个月和12个月时，DC组大鼠白内障的发生率分别为20%、60%和100%。早期胰腺移植（2周）显著降低了这种并发症的发生率，但晚期（12周）胰腺移植则未降低。与DC组和PT2组相比，PT1组大鼠视网膜浅、深毛细血管丛以及Müller细胞的超微结构也有所改善。在最后一组中，尽管胰腺移植成功，但视网膜病变仍在继续发展。