Adam Stacey J, Counter Christopher M
Department of Pharmacology and Molecular Cancer Biology, Duke University, Durham, North Carolina, USA.
Methods Enzymol. 2008;439:39-51. doi: 10.1016/S0076-6879(07)00404-1.
As a biomedical model, pigs offer many advantages and hence have been utilized extensively for toxicology, Crohn's disease, diabetes, and organ transplantation, as well as many other research areas. However, the advantages of porcine models, particularly its large size and similarity to humans, were not exploited previously to any large degree for cancer research. One reason for this lack of porcine cancer models was the inability to induce cancer in pigs genetically. This chapter describes a rapid, reproducible, and genetically malleable method to induce large tumors in pigs.
作为一种生物医学模型，猪具有许多优势，因此已被广泛应用于毒理学、克罗恩病、糖尿病、器官移植以及许多其他研究领域。然而，猪模型的优势，特别是其体型大以及与人类的相似性，以前在癌症研究中并未得到很大程度的利用。缺乏猪癌症模型的一个原因是无法在基因上诱导猪患癌症。本章描述了一种快速、可重复且基因可塑性强的方法，用于在猪体内诱导大型肿瘤。
