Kubán Pavel, Kubán Petr, Kubán Vlastimil, Hauser Peter C, Bocek Petr
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veverí 97, CZ-60200 Brno, Czech Republic.
J Chromatogr A. 2008 May 9;1190(1-2):377-82. doi: 10.1016/j.chroma.2008.03.022. Epub 2008 Mar 13.
In this paper capillary electrochromatography of alkali and alkaline-earth metal cations in open tubular capillary columns is described. Capillary columns are prepared by coating fused silica capillaries of 75 microm I.D. with poly(butadiene-maleic acid) copolymer (PBMA) in multiple layers. Thermally initiated radical polymerization is used to crosslink the stationary phase. Capillary columns with different number of stationary phase layers can be prepared and allow for the adjustment of separation selectivity in the electrochromatographic mode. Fast and sensitive separations of common inorganic cations are achieved in less than 6 min in a 60 cm capillary column with on-column capacitively coupled contactless conductivity detector. Limits of detection (S/N=3) for the determination of alkali and alkaline-earth metal cations range from 0.3 to 2.5 microM and repeatability is better than 0.5, 4.5 and 6.1% for migration times, peak heights and peak areas, respectively.
本文描述了在开口管毛细管柱中进行碱金属和碱土金属阳离子的毛细管电色谱法。毛细管柱通过用聚（丁二烯 - 马来酸）共聚物（PBMA）多层涂覆内径为75微米的熔融石英毛细管来制备。热引发自由基聚合用于交联固定相。可以制备具有不同固定相层数的毛细管柱，并允许在电色谱模式下调节分离选择性。使用柱上电容耦合非接触式电导检测器，在60厘米的毛细管柱中不到6分钟即可实现常见无机阳离子的快速灵敏分离。测定碱金属和碱土金属阳离子的检测限（S/N = 3）范围为0.3至2.5微摩尔，迁移时间、峰高和峰面积的重复性分别优于0.5%、4.5%和6.1%。
