Frazier D E, Tarr M J, Olsen R G
Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
Immunopharmacol Immunotoxicol. 1991;13(1-2):25-46. doi: 10.3109/08923979109019689.
1,1-Dimethylhydrazine or unsymmetrical dimethylhydrazine (UDMH) is a highly volatile and reactive compound used primarily as a liquid rocket propellant. Previous studies found UDMH to possess immunomodulatory activity similar to other hydrazine derivatives. Modulation of T lymphocyte subpopulations and Major Histocompatibility Complex Class II or Ia antigen were evaluated as possible mechanisms for this UDMH-induced immunomodulation. Murine lymphoid cell populations were examined by flow cytometry for changes in their cell surface marker percentages or relative number upon exposure to UDMH either in vitro or in vivo. The results show UDMH caused significant suppression of the T helper cell population derived from the thymus at the 75 mg/kg dose in vivo, but did not affect other lymphocyte subpopulations isolated from mesenteric lymph node, spleen or thymus at this or any other dose. In vivo exposure of mice at all doses of UDMH did not significantly alter expression of Ia antigens on adherent cell populations and expression of the Ia antigen following in vitro UDMH exposure was not affected as well. Results indicate that the immunomodulatory effects of UDMH are not mediated by phenotypic alteration of T lymphocyte subpopulations or Ia antigen.
1,1-二甲基肼或不对称二甲基肼（UDMH）是一种高度挥发性和反应性的化合物，主要用作液体火箭推进剂。先前的研究发现，UDMH具有与其他肼衍生物相似的免疫调节活性。对T淋巴细胞亚群和主要组织相容性复合体II类或Ia抗原的调节被评估为UDMH诱导这种免疫调节的可能机制。通过流式细胞术检查小鼠淋巴细胞群体，以观察其在体外或体内暴露于UDMH后细胞表面标志物百分比或相对数量的变化。结果表明，在体内75mg/kg剂量下，UDMH对源自胸腺的辅助性T细胞群体有显著抑制作用，但在此剂量或任何其他剂量下，对从肠系膜淋巴结、脾脏或胸腺分离的其他淋巴细胞亚群没有影响。在所有剂量的UDMH下，小鼠体内暴露均未显著改变贴壁细胞群体上Ia抗原的表达，体外UDMH暴露后Ia抗原的表达也未受影响。结果表明，UDMH的免疫调节作用不是由T淋巴细胞亚群或Ia抗原的表型改变介导的。
