Kadirvel R, Sundaram K, Mani S, Samuel S, Elango N, Panneerselvam C
Department of Medical Biochemistry, Dr. AL Mudhaliar PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India.
Hum Exp Toxicol. 2007 Dec;26(12):939-46. doi: 10.1177/0960327107087909.
Contamination of arsenic in drinking water is associated with several human diseases including cancer. It has been reported that oxidative stress plays a vital role in arsenic-induced biochemical and molecular alterations. The aim of the present study was to improve the understanding of arsenic-induced oxidative damage to proteins and to DNA and the role of antioxidants such as ascorbic acid and alpha-tocopherol in alleviating arsenic-induced damages in experimental rats. A significant increase in the levels of protein oxidation, DNA strand breaks, and DNA-protein cross-links was observed in blood, liver, and kidney of rats exposed to arsenic (100 ppm in drinking water) for 30 days. Co-administration of ascorbic acid and alpha-tocopherol to arsenic-exposed rats showed a substantial reduction in the levels of arsenic-induced oxidative products of protein and DNA. The results of this study support that free radical-mediated toxic manifestations of arsenic and also suggest that ascorbic acid and alpha-tocopherol supplementation can improve the arsenic-induced molecular alterations.
饮用水中的砷污染与包括癌症在内的多种人类疾病有关。据报道，氧化应激在砷诱导的生化和分子改变中起着至关重要的作用。本研究的目的是增进对砷诱导的蛋白质和DNA氧化损伤以及抗氧化剂（如抗坏血酸和α-生育酚）在减轻实验大鼠砷诱导损伤中的作用的理解。在饮用含砷（饮用水中100 ppm）30天的大鼠的血液、肝脏和肾脏中，观察到蛋白质氧化、DNA链断裂和DNA-蛋白质交联水平显著增加。对砷暴露大鼠同时给予抗坏血酸和α-生育酚，可使砷诱导的蛋白质和DNA氧化产物水平大幅降低。本研究结果支持砷的自由基介导的毒性表现，也表明补充抗坏血酸和α-生育酚可改善砷诱导的分子改变。
