Woźniakiewicz M, Wietecha-Posłuszny R, Garbacik A, Kościelniak P
Laboratory for Forensic Chemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
J Chromatogr A. 2008 May 9;1190(1-2):52-6. doi: 10.1016/j.chroma.2008.03.013. Epub 2008 Mar 18.
A microwave-assisted extraction (MAE) method has been developed and optimized for the extraction of six tricyclic antidepressants (TCAD; nordoxepin, nortriptyline, imipramine, amitriptyline, doxepin, dezypramine) from human serum. Optimal parameters of MAE (solvent and extraction temperature) for water solution of these drugs were defined. The microwave-assisted procedure developed was validated by extraction of serum samples at two concentration levels and then successfully applied to the analysis of reference material. Limit of quantification, precision and recovery were found for the studied compounds in the ranges 0.04-0.15 microg/mL, 1.57-4.34% (RSD) and 94-105%, respectively.
已开发并优化了一种微波辅助萃取（MAE）方法，用于从人血清中萃取六种三环类抗抑郁药（TCAD；去甲多虑平、去甲替林、丙咪嗪、阿米替林、多塞平、地昔帕明）。确定了这些药物水溶液的MAE最佳参数（溶剂和萃取温度）。通过在两个浓度水平萃取血清样品对所开发的微波辅助程序进行了验证，然后成功应用于参考物质的分析。所研究化合物的定量限、精密度和回收率分别在0.04 - 0.15微克/毫升、1.57 - 4.34%（相对标准偏差）和94 - 105%范围内。
