Thomas Peter, Sweatman Jennifer
University of Texas at Austin, Marine Science Institute, Port Aransas, TX 78373, USA.
Mar Environ Res. 2008 Jul;66(1):1-2. doi: 10.1016/j.marenvres.2008.02.005. Epub 2008 Feb 26.
Recent studies have shown that several environmental chemicals which disrupt classical genomic steroid actions can also interfere with nongenomic steroid actions initiated at the cell surface. The effects of bisphenol-A and atrazine on the nongenomic actions of a progestin, 17,20 beta,21-trihydroxy-4-pregnen-3-one (20 beta-S), on oocyte maturation (OM) were investigated an in vitro Atlantic croaker (Micropogonias undulatus) oocyte bioassay. Treatment of croaker follicle-enclosed oocytes with either bisphenol-A or atrazine blocked OM in response to 20 beta-S in a concentration-dependent manner at 10-25 microM (2.2-5.7 ppm) and higher concentrations. These compounds were also effective competitors at concentrations of 10(-6)-10(-5)M of [3H]-20 beta-S binding to the ovarian progestin membrane receptor that mediates the OM response to 20 beta-S. The results suggest that diverse classes of environmental chemicals can disrupt nongenomic progestin actions via receptor-mediated mechanisms.
近期研究表明，几种干扰经典基因组类固醇作用的环境化学物质，也能干扰在细胞表面启动的非基因组类固醇作用。利用体外大西洋鲳鱼（Micropogonias undulatus）卵母细胞生物测定法，研究了双酚A和阿特拉津对孕激素17,20β,21-三羟基-4-孕烯-3-酮（20β-S）非基因组作用于卵母细胞成熟（OM）的影响。用双酚A或阿特拉津处理鲳鱼卵泡包被的卵母细胞，在10-25微摩尔/升（2.2-5.7 ppm）及更高浓度下，以浓度依赖方式阻断了对20β-S的卵母细胞成熟反应。在10⁻⁶-10⁻⁵摩尔/升浓度下，这些化合物也是[³H]-20β-S与介导卵母细胞成熟对20β-S反应的卵巢孕激素膜受体结合的有效竞争者。结果表明，不同种类的环境化学物质可通过受体介导机制干扰非基因组孕激素作用。
