基于β-咔啉的非离子型探针在阴离子和两性离子脂质体膜中的光物理性质
Photophysics of a beta-carboline based non-ionic probe in anionic and zwitterionic liposome membranes.
作者信息
Das Paramita, Sarkar Deboleena, Chattopadhyay Nitin
机构信息
Department of Chemistry, Jadavpur University, Calcutta 700032, India.
出版信息
Chem Phys Lipids. 2008 Jul;154(1):38-45. doi: 10.1016/j.chemphyslip.2008.03.003. Epub 2008 Mar 13.
Interaction of a biologically active beta-carboline based non-ionic probe, 3-acetyl-4-oxo-6,7-dihydro-12H indolo-[2,3-a] quinolizine (AODIQ), with the liposomal vesicles of dimyristoyl-l-alpha-phosphatidylcholine (DMPC) and dimyristoyl-l-alpha-phosphatidylglycerol (DMPG) has been demonstrated using steady-state and time-resolved fluorescence and fluorescence anisotropy techniques. Polarity sensitive intramolecular charge transfer of AODIQ shows a large hypsochromic shift along with an enhancement in the fluorescence quantum yield and fluorescence lifetime in the bilayer membranes compared to those in aqueous buffer solution. Polarity of the immediate vicinity of the probe in the lipid environments has been determined. The fluorometric, quenching and micropolarity determination studies reveal that the fluorophore penetrates deeper in the zwitterionic DMPC membrane compared to the anionic DMPG vesicle. Enhancement in the rotational relaxation time of AODIQ in liposomal membranes suggests that the fluorophore exists in motionally restricted environments.
使用稳态和时间分辨荧光以及荧光偏振技术，已证明一种基于生物活性β-咔啉的非离子探针3-乙酰基-4-氧代-6,7-二氢-12H吲哚并-[2,3-a]喹嗪（AODIQ）与二肉豆蔻酰基-l-α-磷脂酰胆碱（DMPC）和二肉豆蔻酰基-l-α-磷脂酰甘油（DMPG）的脂质体囊泡之间存在相互作用。与在水性缓冲溶液中相比，AODIQ的极性敏感分子内电荷转移在双层膜中显示出较大的蓝移，同时荧光量子产率和荧光寿命增强。已确定脂质环境中探针紧邻区域的极性。荧光测定、猝灭和微极性测定研究表明，与阴离子型DMPG囊泡相比，荧光团在两性离子型DMPC膜中渗透得更深。AODIQ在脂质体膜中旋转弛豫时间的增加表明荧光团存在于运动受限的环境中。
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