Yan Zhenguang, Ma Zhuojun, Zheng Guilan, Feng Qiaoli, Wang Hongzhong, Xie Liping, Zhang Rongqing
Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Chembiochem. 2008 May 5;9(7):1093-9. doi: 10.1002/cbic.200700553.
In mollusks, the inner shell film is located in the shell-mantle zone and it is important in shell formation. In this study, we found that the film was composed of two individual films under certain states and some columnar structures were observed between the two individual films. The inner shell film was separated with the process of ethylenediaminetetraacetic acid (EDTA) treatment and the film proteins were extracted. Amino acid analysis showed that the film proteins may consist of shell framework proteins. The calcite crystallization experiment showed that the film proteins could inhibit the growth of calcite, while the CaCO(3) precipitation experiment showed that the film proteins could accelerate the rate of CaCO(3) precipitation. All these results suggested that the film plays an important role in shell formation. It may facilitate the aragonite formation by inhibiting the growth of calcite and accelerate the shell growth by promoting the precipitation of CaCO(3) crystals.
在软体动物中，内壳膜位于壳-外套膜区域，在贝壳形成过程中起着重要作用。在本研究中，我们发现该膜在特定状态下由两层独立的膜组成，并且在这两层独立的膜之间观察到一些柱状结构。通过乙二胺四乙酸（EDTA）处理过程将内壳膜分离，并提取膜蛋白。氨基酸分析表明，膜蛋白可能由贝壳框架蛋白组成。方解石结晶实验表明，膜蛋白可以抑制方解石的生长，而碳酸钙沉淀实验表明，膜蛋白可以加速碳酸钙的沉淀速率。所有这些结果表明，该膜在贝壳形成过程中起着重要作用。它可能通过抑制方解石的生长来促进文石的形成，并通过促进碳酸钙晶体的沉淀来加速贝壳的生长。
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