Glover Joel C, Sato Katsushige, Momose-Sato Yoko
Department of Physiology, University of Oslo, PB 1103 Blindern, Oslo, Norway.
Dev Neurobiol. 2008 May;68(6):804-16. doi: 10.1002/dneu.20629.
Recent developments in the design of voltage-sensitive dyes and of recording apparatuses for detecting voltage-dependent changes in the optical properties of such dyes have established voltage-sensitive dye recording as an important technique for assessing the functional development of neuronal circuits in the brain and spinal cord. Here we discuss general technical issues regarding the recording of voltage-sensitive dye signals and describe studies that have utilized this approach to follow the development of sensory and sensorimotor circuits in the embryonic brain stem. Functional imaging through voltage-sensitive dye recording permits a noninvasive analysis of synaptic development and function at submillisecond temporal resolution in widely distributed circuits. These advantages are particularly valuable in assessing sensorimotor circuit development at early stages when neurons are small and synapses are fragile.
电压敏感染料的设计以及用于检测此类染料光学特性中电压依赖性变化的记录设备的最新进展，已将电压敏感染料记录确立为评估大脑和脊髓中神经回路功能发育的一项重要技术。在此，我们讨论与电压敏感染料信号记录相关的一般技术问题，并描述利用这种方法追踪胚胎脑干中感觉和感觉运动回路发育的研究。通过电压敏感染料记录进行的功能成像能够以亚毫秒级的时间分辨率对广泛分布的回路中的突触发育和功能进行无创分析。这些优势在评估神经元较小且突触脆弱的早期感觉运动回路发育时尤为重要。
