Ebner T J, Chen G
Department of Neurosurgery, University of Minnesota, Minneapolis 55455, USA.
Prog Neurobiol. 1995 Aug;46(5):463-506. doi: 10.1016/0301-0082(95)00010-s.
Understanding the spatio-temporal features of the information processing occurring in any complex neural structure requires the monitoring and analysis of the activity in populations of neurons. Electrophysiological and other mapping techniques have provided important insights into the function of neural circuits and neural populations in many systems. However, there remain limitations with these approaches. Therefore, complementary techniques which permit the monitoring of the spatio-temporal activity in neuronal populations are of continued interest. One promising approach to monitor the electrical activity in populations of neurons or on multiple sites of a single neuron is with voltage-sensitive dyes coupled with optical recording techniques. This review concentrates on the use of voltage-sensitive dyes and optical imaging as tools to study the activity in neuronal populations in the central nervous system. Focusing on 'fast' voltage-sensitive dyes first, several technical issues and developments in optical imaging will be reviewed. These will include more recent developments in voltage-sensitive dyes as well as newer developments in optical recording technology. Second, studies using voltage-sensitive dyes to investigate information processing questions in the central nervous system and in the invertebrate nervous system will be reviewed. Some emphasis will be placed on the cerebellum, but the major goal is to survey how voltage-sensitive dyes and optical recordings have been utilized in the central nervous system. The review will include optical studies on the visual, auditory, olfactory, somatosensory, auditory, hippocampal and brainstem systems, as well as single cell studies addressing information processing questions. Discussion of the intrinsic optical signals is also included. The review attempts to show how voltage-sensitive dyes and optical recordings can be used to obtain high spatial and temporal resolution monitoring of neuronal activity.
要理解任何复杂神经结构中发生的信息处理的时空特征，需要监测和分析神经元群体的活动。电生理和其他映射技术为许多系统中神经回路和神经群体的功能提供了重要见解。然而，这些方法仍然存在局限性。因此，能够监测神经元群体时空活动的互补技术一直备受关注。一种有前景的监测神经元群体或单个神经元多个部位电活动的方法是使用电压敏感染料结合光学记录技术。本综述集中讨论电压敏感染料和光学成像作为研究中枢神经系统中神经元群体活动的工具的应用。首先关注“快速”电压敏感染料，将回顾光学成像中的几个技术问题和进展。这些将包括电压敏感染料的最新进展以及光学记录技术的新发展。其次，将回顾使用电压敏感染料研究中枢神经系统和无脊椎动物神经系统中信息处理问题的研究。将重点关注小脑，但主要目标是概述电压敏感染料和光学记录在中枢神经系统中的应用方式。综述将包括对视觉、听觉、嗅觉、体感、听觉、海马体和脑干系统的光学研究，以及解决信息处理问题的单细胞研究。还将讨论内在光学信号。本综述试图展示电压敏感染料和光学记录如何用于获得对神经元活动的高空间和时间分辨率监测。
