[使用两种自旋标记研究肌球蛋白的构象转变]
[Study of conformational transitions in myosin using 2 types of spin label].
作者信息
Ruuge E K, Medvedeva N V, Vilenkina M N, Bliumenfel'd L A
出版信息
Biofizika. 1976 May-Jun;21(3):409-12.
PMID:183836
Abstract
It is shown that three types of ESR spectra of myosin labeled with both acetate and acetamide spin labels are distinguished. These three types are the spectrum of initial preparation, myosin--ADP complex spectrum and the spectrum recorded during ATP hydrolysis. The ESR spectra of myosin labeled with acetate spin label are more sensitive to conformational changes of myosin induced by complex formation and temperature.
摘要
结果表明，可以区分用乙酸盐和乙酰胺自旋标记物标记的肌球蛋白的三种类型的电子自旋共振（ESR）光谱。这三种类型分别是初始制剂的光谱、肌球蛋白 - ADP复合物光谱以及ATP水解过程中记录的光谱。用乙酸盐自旋标记物标记的肌球蛋白的ESR光谱对由复合物形成和温度诱导的肌球蛋白构象变化更敏感。
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