Wada Nobuyuki, Yoshida Akira, Miyagi Yohei, Yamamoto Toshiharu, Nakayama Hirotaka, Suganuma Nobuyasu, Matsuzu Kenichi, Masudo Katsuhiko, Hirakawa Shohei, Rino Yasushi, Masuda Munetaka, Imada Toshio
Department of General Surgery, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama City, Kanagawa, Japan.
Anticancer Res. 2008 Jan-Feb;28(1A):139-44.
The mitotic spindle assembly checkpoint (MSAC) genes are responsible for preventing chromosome missegregation. MSAC gene expressions have been reported to be associated with tumor cell proliferation or unfavorable cancer behavior. The present study was conducted to preliminary investigate the MSAC gene expressions in thyroid neoplasms.
Expression levels of MSAC genes (hBUB1, hBUBR1, hBUB3 and hMAD2) were evaluated in 9 follicular thyroid adenomas (FAs), 9 follicular thyroid carcinomas (FTCs), 21 papillary thyroid carcinomas (PTCs), 5 anaplastic (undifferentiated) thyroid carcinomas (ATCs) and 3 adjacent normal thyroid tissues (NTs) by real-time quantitative RT-PCR. These gene expressions were compared between undifferentiated thyroid carcinomas (ATCs) and differentiated thyroid carcinomas (DTCs) and between advanced DTCs and non-advanced DTCs. DTCs included PTCs and FTCs. Advanced DTCs were defined as carcinoma with aggressive nature such as extrathyroid extension, distant metastasis, recurrence or death from the disease.
MSAC gene expressions varied in different thyroid tumors and fell in the order of ATC, DTC (PTC and FTC), FA and NT Carcinomas had higher expression compared to adenoma or normal tissue. hBUB1, hBUBR1 and hMAD2 expressions in ATCs were significantly higher than those in DTCs (p<0.005). hBUBR1 and hMAD2 expressions in advanced DTCs were significantly higher than those in non-advanced DTCs (p<0.05).
The MSAC genes were overexpressed in thyroid carcinomas with aggressive nature. Further studies are required to clarify the relationship between the MSAC gene expressions and thyroid cancer behavior.
有丝分裂纺锤体组装检查点（MSAC）基因负责防止染色体错分离。据报道，MSAC基因表达与肿瘤细胞增殖或不良癌症行为有关。本研究旨在初步调查甲状腺肿瘤中MSAC基因的表达情况。
通过实时定量逆转录聚合酶链反应（RT-PCR）评估9例甲状腺滤泡性腺瘤（FA）、9例甲状腺滤泡癌（FTC）、21例甲状腺乳头状癌（PTC）、5例间变性（未分化）甲状腺癌（ATC）和3例相邻正常甲状腺组织（NT）中MSAC基因（hBUB1、hBUBR1、hBUB3和hMAD2）的表达水平。比较未分化甲状腺癌（ATC）与分化型甲状腺癌（DTC）之间以及晚期DTC与非晚期DTC之间的这些基因表达。DTC包括PTC和FTC。晚期DTC定义为具有侵袭性的癌症，如甲状腺外侵犯、远处转移、复发或死于该疾病。
MSAC基因表达在不同甲状腺肿瘤中有所不同，顺序为ATC、DTC（PTC和FTC）、FA和NT。癌组织的表达高于腺瘤或正常组织。ATC中hBUB1、hBUBR1和hMAD2的表达明显高于DTC（p<0.005）。晚期DTC中hBUBR1和hMAD2的表达明显高于非晚期DTC（p<0.05）。
MSAC基因在具有侵袭性的甲状腺癌中过表达。需要进一步研究以阐明MSAC基因表达与甲状腺癌行为之间的关系。
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