人喉癌中与分期相关的核心蛋白聚糖和多功能蛋白聚糖表达

Stage-related decorin and versican expression in human laryngeal cancer.

作者信息

Stylianou M, Skandalis S S, Papadas T A, Mastronikolis N S, Theocharis D A, Papageorgakopoulou N, Vynios D H

机构信息

Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26500 Patras, Greece.

出版信息

Anticancer Res. 2008 Jan-Feb;28(1A):245-51.

PMID:18383852

Abstract

BACKGROUND

The major proteoglycan of normal human larynx is aggrecan. In laryngeal carcinoma, aggrecan is depleted, with versican and decorin appearing in higher amounts.

MATERIALS AND METHODS

Proteoglycans in laryngeal carcinoma samples were characterized immunohistochemically and using Western blotting; their expression was examined by RT-PCR.

RESULTS

Aggrecan was totally removed in advanced cancer and its RT-PCR product was not identified. Both versican and decorin were overexpressed in cancer, versican much more than decorin. Decorin expression was higher than that of versican in the normal larynx; therefore, their disproportionate overexpression during cancer resulted in about equimolar expression. Both proteoglycans' expression correlated with their stage-related accumulation within the tissue.

CONCLUSION

These data add to our previous findings and support the view that the levels of expression and the extent of accumulation and localization in the tumor stroma of structurally modified versican and decorin could be associated with the degree of aggressiveness of laryngeal carcinoma.

摘要

背景

正常人喉的主要蛋白聚糖是聚集蛋白聚糖。在喉癌中，聚集蛋白聚糖减少，而多功能蛋白聚糖和饰胶蛋白聚糖的含量增加。

材料与方法

采用免疫组织化学和蛋白质印迹法对喉癌样本中的蛋白聚糖进行表征；通过逆转录聚合酶链反应（RT-PCR）检测它们的表达。

结果

在晚期癌症中聚集蛋白聚糖完全消失，且未鉴定出其RT-PCR产物。多功能蛋白聚糖和饰胶蛋白聚糖在癌症中均过度表达，多功能蛋白聚糖的过度表达程度远高于饰胶蛋白聚糖。在正常喉中，饰胶蛋白聚糖的表达高于多功能蛋白聚糖；因此，它们在癌症期间不成比例的过度表达导致了大约等摩尔表达。两种蛋白聚糖的表达均与其在组织内与阶段相关的积累相关。