层粘连蛋白聚糖与膀胱癌的肿瘤进展、转移和不良预后相关。
Upregulation of Versican Associated with Tumor Progression, Metastasis, and Poor Prognosis in Bladder Carcinoma.
机构信息
Department of Urology, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou 310014, China.
Graduate Department, Zhejiang Chinese Medical University, Hangzhou 310053, China.
出版信息
Biomed Res Int. 2021 Feb 2;2021:6949864. doi: 10.1155/2021/6949864. eCollection 2021.
OBJECTIVE
This work analyzes the role of versican (VCAN) on bladder cancer (BLCA). Versican (VCAN) is a chondroitin sulfate proteoglycan which is important for tumorigenesis and the development of cancer. However, the expression of VCAN on human bladder cancer (BLCA) has been rarely reported.
METHODS
The clinical significance of VCAN in BLCA has been determined by our bioinformatics tools. Then, we performed immunohistochemical staining (IHC) and analyzed the correlation between VCAN expression and clinicopathological features.
RESULTS
The bioinformatics results reveal that a high VCAN mRNA level was significantly associated with stage, histological subtype, molecular subtype, and metastasis in BLCA. Furthermore, IHC reveals that expression of VCAN was significantly correlated with the number of tumors, invasion depth, lymph node metastasis, distant metastasis, and histological grade. Kaplan-Meier survival analysis reveals that patients with a high expression of VCAN have poor prognosis than those patients with a low expression of VCAN. According to our result from the bioinformatics database, the mechanism of VCAN in BLCA revealed that VCAN was related to FBN1 and genes of the ECM remodeling pathway (MMP1, MMP2).
CONCLUSION
VCAN expression might be included in the process of carcinogenesis and prognosis. Hence, VCAN could be a reliable biomarker of the clinical prognosis on BLCA.
目的
本研究分析了 versican（VCAN）在膀胱癌（BLCA）中的作用。VCAN 是一种软骨素硫酸蛋白聚糖，对肿瘤发生和癌症发展很重要。然而，VCAN 在人膀胱癌（BLCA）中的表达却鲜有报道。
方法
我们的生物信息学工具确定了 VCAN 在 BLCA 中的临床意义。然后，我们进行了免疫组织化学染色（IHC）分析，并分析了 VCAN 表达与临床病理特征之间的相关性。
结果
生物信息学结果表明，VCAN mRNA 水平高与 BLCA 的分期、组织学亚型、分子亚型和转移显著相关。此外，IHC 显示 VCAN 的表达与肿瘤数量、浸润深度、淋巴结转移、远处转移和组织学分级显著相关。Kaplan-Meier 生存分析显示，VCAN 高表达的患者预后比 VCAN 低表达的患者差。根据我们从生物信息学数据库得到的结果，VCAN 在 BLCA 中的作用机制表明，VCAN 与 FBN1 和 ECM 重塑途径的基因（MMP1、MMP2）有关。
结论
VCAN 的表达可能参与了膀胱癌的癌变和预后过程。因此，VCAN 可能是 BLCA 临床预后的可靠标志物。
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